WebThereby, GSH assay (Thiol Tracker Violet fluorescent dye) was used to investigate the cascade enzymatic activity of PCF-a NEs (Figure 3b). The GSH level in the control group of 4T1 cells showed... WebDec 2, 2024 · Changes in intracellular thiol groups pool were evaluated using thiol tracker violet. Angeli’s salt was used as HNO donor. Results The HNO donor Angeli’s salt induced a significant increase in the cytosolic Ca 2+ concentration at the concentration 50 µM and a membrane hyperpolarization from a resting membrane potential of −56.1 ± 8.0 mV ...
Detection of Intracellular Glutathione - ResearchGate
WebMay 28, 2004 · Here we further studied the mechanism of parthenolide-induced apoptosis by focusing on the role of intracellular thiols and calcium in a human colorectal cancer cell, COLO 205. Parthenolide rapidly depleted intracellular thiols, including both free glutathione (GSH) and protein thiols. WebDescription. ThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority of … pop up cinema erith
Molecular Probes™ ThiolTracker™ Violet (glutathione detection …
WebThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority... Molecular Probes™ … WebDec 14, 2024 · Measurement of reduced (free) thiol with thiol tracker. Cells (0.1 × 10 6) were stained in 1 µM Thiol tracker Violet (Life technologies) for 30 min at 37°C. Cells were … WebMar 20, 2024 · As observed under the confocal microscopy, 4T1 cells treated by free BSO and [email protected] 3-PEG (BSO = 200 μM, Ca 2+ = 4 mM) and stained with Thiol Tracker violet dye displayed remarkably reduced green fluorescence inside cells compared with cells in the control group and cells treated with plain [email protected] 3-PEG (Figure 3D). sharon leptin