Peasy-t5 zero cloning kit
WebJan 22, 2024 · The product was cloned into the pEASY-T5 Zero Cloning vector (TransGene Biotech, Beijing, China) for sequencing. For 5′ RACE, the gene specific primers were designed based on the 3′ cDNA end sequence. The 5′ RACE cDNA template was synthesized by SMARTer@RACE cDNA Amplification Kit (TaKaRa, Tokyo, Japan). WebFree Trial pEASY-Blunt Simple (linearized) Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter but …
Peasy-t5 zero cloning kit
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WebFeatures of the pEASY-Blunt Zero Cloning Kit. 5 minutes fast insertion of blunt-end PCR products.by topoisomerase. High cloning efficiency. Positive clones up to 100%. No blue/white selection needed. Suitable for larger … WebFeb 15, 2024 · 分别以杜梨cDNA和gDNA为模板扩增PbGID1家族基因。扩增程序为：95 ℃预变性2 min；95 ℃ 30 s，55 ℃ 30 s，72 ℃ 1 min，30个循环；72 ℃ 延伸 2 min。PCR产物进行胶回收后，连接至pEASY-Blunt Zero Cloning Kit克隆载体，转化大肠杆菌感受态DH5α中，挑取单克隆测序。
WebTransGen Biotech pEASY®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector … WebMay 20, 2024 · The PCR products obtained were purified and ligated into the vector pEASY -T5 and subsequently recombinant plasmids were used to transform E. coli Trans 1-T1 competent cells using a pEASY -T5 Zero Cloning Kit (TransGen Biotech, Beijing, China). Both of the recombinant plasmids were verified by sequencing.
WebpEASY-Blunt Zero (linearized) Cloning vector for blunt-ended PCR products, with a lacZα-ccdB suicide gene that allows for selection of recombinant plasmids. Sequence Author: … WebJul 30, 2024 · Recombination plasmid of HA gene of H5 subtype has been constructed using the pEASY™-T5 Zero Cloning Kit. The plasmid was cleaved using a suitable restriction enzyme. The digested plasmid was purified with a DNA recovery kit, the concentration was measured with a Nano Drop nucleic acid quantifier, and then an appropriate amount was …
WebpEASY-T5 Zero Cloning Kit (TransGen Biotech, Beijing, China). Both of the recombinant plasmids were verified by sequencing. All the sequences of the primers used in this study are shown in
WebThe optimal vector-to-insert molar ratio is 1:7. · It can be roughly calculated at the ratio of "1 kb- to-20 ng" (e. g. 20ng for 1 kb inserted DNA, ; 30 ng for 1.5 kb inserted DNA) (The amount can be quantified roughly by TransGen's DNA molecular weight standard on the gel.) · The maximum amount of fragment addition is 4 μl, and do not ... how to get to kronos the final justiceWebThe recovered PCR products were cloned using the pEASY-T5 Zero Cloning Kit (TransGen Biotech (Beijing) Co., Ltd, Beijing, China) by following the product manual. Two positive final clones were submitted to Invitrogen Corporation (Guangzhou, China) for sequencing. Taq 2X PCR Master Mix, DNA Marker, deoxyribonucleoside triphosphates (dNTPs) and ... johns furniture removals yandinaWebTransGen biotech co peasy t5 zero cloning kit Peasy T5 Zero Cloning Kit, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 88/100, based on 1 … how to get to kronos of the past shadovis rpgWebpEASY cloning kits a proprietary topoisomerase (TOPO) enzyme blend to efficiently clone your DNA in any of the pEASY vectors in only 5 minutes. ... pEASY-Blunt Zero Cloning Kit – CB501 $ 338.00 – $ 898.00 CAD; pEASY-Blunt3 Cloning Kit – CB301 ... pEASY®-T5 Zero Cloning Kit – CT501 $ 338.00 – $ 673.00 CAD; Filter by Supplier. Most ... how to get to krokuun from orgrimmarWebpEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed … how to get to kraid in metroid dreadWebJan 8, 2016 · The recovered PCR products were cloned using the pEASY-T5 Zero Cloning Kit (TransGen Biotech Co., Ltd, Beijing, China) by following the product manual. Two positive final clones were submitted to Invitrogen Corporation (Guangzhou, China) for sequencing. Prokaryotic Expression and SDS-PAGE Analysis. how to get to krosus nightholdWebOct 4, 2024 · Each PCR product was inserted into the pEASY-T5 Zero Cloning Vector (TransGen Biotech, Beijing), and more than five clones per reaction were sequenced (Ykang, Hangzhou, China). The 5’ and 3’RACE fragments amplified using the corresponding primers were then assembled using DNAMAN 8.0 software (Lynnon Biosoft, Canada). johns frosted mug forsyth