WebTable 1. Reaction Component Amounts Recommended for Scaling Transfections Up or Down. Tube 1 Tube 2 Culture Vessel 1 1X Xfect Protein Transfection Reagent Stock (See Section II.A) Deionized Water Your Protein1 Xfect Protein Buffer Serum-Free Medium2 96-well plate 0.75 μl 4.25 μl 0.1–0.25 μg to 5 μl 40 μl Web28 jun. 2016 · The reporter vector without a miRNA binding sequences was used as a control. 3′-UTR-luciferase reporter constructs were transfected into cranial NCCs using …
MegaTran 2.0, plasmid DNA transfection reagent (1 mL)
WebLipoD293™ reagent (Ver. II), 1.0 ml, is sufficient for ~666 transfections in 24 well plates or ~333 transfections in 6 well plates. Figure 1. A Cartoon Showing LipoD293™ (Ver. II) Enhanced Gene Delivery Features - Top choice for hard-to-transfect cells - Exceptional high titers of virus production - Equally good for very long DNAs (up to 180 kb) Web2.1 Transfection of adherent cells – Standard protocol for a 12- well plate - 1. In a 12-well tissue culture plate, seed 1.0 – 4.0 x 105(starting point: 2.0 x 105) cells per dish in 1 ml of suitable fresh complete medium.2 2. Incubate the cells at 37°C in a CO2incubator until growing area is 90–100% covered. dyson airwrap short hair review
MegaTran 2.0, plasmid DNA transfection reagent (5 X 1 mL)
WebPolyJet™ In Vitro DNA Transfection Reagent (1.0 ml) DescriptionBased on our proprietary polymer synthesis technology, PolyJet™ DNA In Vitro Transfection Reagent is formulated as a biodegradable polymer based DNA transfection reagent that ensures effective and reproducible transfection on HEK293, COS-7, NIH-3T3, HeLa, CHO and a broad ra Menu WebD. TransIT®-LT1 Reagent to DNA ratio - As a starting point, use 3 µl of TransIT®-LT1 Reagent per 1 µg of DNA. Titrate the TransIT®-LT1 Reagent from 2-8 µl per 1 µg DNA, … WebX-tremeGENE ™ 360 Transfection Reagent is an innovative reagent that forms a complex with DNA or RNA and efficiently delivers the nucleic acids into animal or insect cells. With improved utility, X-tremeGENE ™ 360 delivers high efficiency transfection in more cell types with low cytotoxicity. 애플리케이션 dyson airwrap sets