Immortalized human podocyte cell line

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WitrynaExperimental approach: Expression of TRPV1-4 channels was investigated at protein (immunocytochemistry, Western blot) and mRNA (Q-PCR) level in a conditionally immortalized human podocyte cell line. Channel function was assessed by measuring intracellular Ca 2+ concentration using Flou-4 Ca 2+ -indicator dye and patch clamp … WitrynaOther investigators have immortalized mouse and human neural stem cells using retroviral transduction of the v-myc oncogene (Snyder, 1995; ... 2007) and his team …

Expression profiles of podocyte-specific marker proteins known as ...

Witryna3 sty 2024 · In this study, conditionally immortalized human podocytes were used, ... This podocyte cell line proliferates under permissive conditions at 33 °C. When cultivated at 37 °C, the SV40 T-antigen is inactivated for cell differentiation. The culture medium for human podocytes was RPMI 1640 Medium (Gibco, via Thermo Fisher … WitrynaThe protein products of these canonical podocyte genes are essential for the proper podocyte function. In cell culture, human and mouse podocytes also lose expression of NPHS1 and NPHS2, 23, 24 ... ipad stuck on ios 12

Direct effects of dexamethasone on human podocytes - PubMed

WitrynaMouse Podocyte Cell Line Mpc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ... Witryna17 wrz 2024 · Generally, conditionally immortalized podocytes have lost many of their phenotypes in vivo, including process formation at high cell density with conventional … WitrynaImmortalized Human Kidney Podocytes (IM-HKP) provided by Innoprot have been developed by immortalizing primary human healthy kidney podocytes with SV40 … open road radio gina woods

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Podocyte culture: Tricks of the trade - NI - Wiley Online …

WitrynaOur normal human primary cells retain in vivo physiologies and custom lots are available from a diverse donor pool. ATCC hTERT-immortalized primary cells also display … WitrynaCell Lines. HK2 cells (ATCC) were cultured in Dulbecco’s Modified Eagle Medium Low Glucose (DMEM LG) supplemented with 10% foetal bovine serum (FBS) (Euroclone S.p.A., Pero, MI, Italy). Immortalized human podocytes, previously characterized were cultured in DMEM High Glucose (Euroclone) with 10% FBS. Glomerular endothelial … open road renewablesWitrynaA conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc Nephrol. 2002; 13: 630-638. Crossref; PubMed; Google Scholar, 20. Saleem M.A. Ni L. Witherden I. et al. Co-localization of nephrin, podocin, and the actin cytoskeleton: evidence for a role in podocyte foot process formation. open road pre owned

"Immortalised cell lines are widely used as a simple model for more complex biological systems, for example for the analysis of the biochemistry and cell biology of mammalian (including human) cells. The main advantage of using an immortal cell line for research is its immortality; the cells can be grown indefinitely in culture. This simplifies analysis of the biology of cells which may otherwise have a limited lifetime. " - Immortalized human podocyte cell line

Immortalized human podocyte cell line

A novel method for successful induction of ... - ScienceDirect

Witryna18 kwi 2024 · The immortalized human podocyte cell line were purchased from Bristol University and cultured under their published protocol. Podocyte mRNA profiles of these cells were examined. Contributor(s) Yoshimura Y, Nishinakamura R: Citation(s) 30635375: Submission date: Jun 30, 2024: Last update date: Apr 18, 2024: WitrynaImmortalized Human Podocytes are podocytes isolated from urine samples from healthy donor, and had been transformation with the thermosensitive SV40 large T …

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WitrynaThe Human Podocyte Cell Line (CIHP-1) is a primary cell line isolated from human kidney. Podocytes are highly specialised, terminally differentiated cells with a complex … WitrynaThe podocyte is a key cell in the glomerulus in health and disease: until recently, human podocytes have been difficult to study in vitro. We have developed a conditionally immortalized human podocyte cell line transfected with a temperature-sensitive simian virus 40 transgene: when the transgene is inactivated in vitro, these …

WitrynaCell line name: CIHP-1: Synonyms: Conditionally Immortalized Human Podocytes-1; AB8/13; AB 8/13; hiPOD; ciPOD: Accession: CVCL_W186: Resource Identification … Witryna11 lis 2009 · Conditionally immortalized human podocytes were cultured for up to 24 h with 375–750 μM palmitate. Functional effects on glucose uptake and ceramide production were measured. ... Reiser J, et al. A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc …

WitrynaImmortalized Human Kidney Podocytes are developed by immortalizing primary human healthy kidney podocytes with SV40 Large T antigen. These kidney podocytes were … WitrynaA seminal breakthrough came about a decade ago with the documentation of differentiation in culture of primary rat and human podocytes and the subsequent development of conditionally immortalized differentiated podocyte cell lines that allow deciphering the decisive steps of differentiation and function of ‘in vivo’ podocytes. …

Witrynaeases. A conditionally immortalized human podocyte cell line has been developed by transfection with the temperature-sen-sitive SV40-Tgene. These cells …

WitrynaATCC cell lines immortalized human podocytes ab8 13 m saleem Cell Lines Immortalized Human Podocytes Ab8 13 M Saleem, supplied by ATCC, used in … open road refinancing for bad creditWitrynaThe human podocytes derived from the stem cells discussed in this review have shown some advantages over the immortalized podocytes, but their maturation remains a question that has not been compared with that of the human adults in-vivo. 42,43,89 Among the various reports on the induction of podocytes and kidney organoids from … ipad stuck on press home button to upgradeWitrynaConditionally immortalized human podocyte WT and CRISPR/CAS9 GLA KO cell lines were differentiated at 37 °C at 70% confluency for 10 to 14 days. Growth protocol Conditionally immortalized human podocytes were let to proliferate at 33°C in RPMI-1640 medium supplemented with 10% fetal calf serum (FCS), Penicillin/Streptomycin, … openroad redrover toonboxWitrynaHuman cell lines are a critical biological resource. These in vitro models of human biology are used in biomedical research, toxicology studies, bioindustry, drug … ipad stuck on home screenWitrynaIn contrast, no significant change was observed in mesangial cells after 12 h, and even after 48 h the wounds were not completely closed. Until now, conditionally … open road renewables llcWitrynaA stable SPL-kd cell line of human podocytes was generated by the lentiviral shRNA transduction method and was characterized for reduced SPL mRNA and protein levels and increased S1P levels. ipad stuck on loading accessories and scenesWitryna1 mar 2010 · The generation of podocytes cell lines from urine of patients and healthy volunteers is novel and will help to advance studies of podocyte cell biology, and further improvements in the approaches to cell transformation and/or cell culture techniques are needed to allow cultured podocytes to fully reproduce in vivo characteristics. … open road psychiatric services