Halolysin

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WebHemolysin is an important virulence factor in the extremely common E. coli infections of the urinary tract (UTI) and other extraintestinal sites [ 4,5,14] and is also produced by … WebMay 23, 1996 · A gene encoding a halophilic serine proteinase, halolysin R4, from a halophilic archaeon Haloferax mediterranei strain R4 was cloned, its nucleotide …

Halolysin R4, a serine proteinase from the halophilic archaeon

WebHalolysin SptA from haloarchaeon Natrinema sp. J7 consists of a subtilisin-like catalytic domain and a C-terminal extension (CTE) containing two cysteine residues. In this report, we have investigated the function of the CTE using recombinant enzymes expressed in Haloferax volcanii WFD11. Deletion of the CTE greatly reduced but did not abolish ... WebAug 3, 2024 · Halolysin SptA is the major extracellular protease of Natrinema sp. J7-2, and its production is growth-phase dependent. The growth phase-dependent production of … hill terrain map

Halolysin SptA, a Serine Protease, Contributes to Growth-Phase ...

WebHalolysin precursors contain a twin arginine signal pep-tide, a propeptide, a subtilase catalytic domain and a C-ter-minal extension (CTE). During or after the trans-membrane transport, they are autocatalytically cleaved to generate a mature halolysin. The mature halolysin is composed of a cat-alytic domain and a CTE (Du et al. 2015). WebJul 29, 2006 · Thus far, the biochemical properties of many halophilic proteases have been studied extensively, while only the genes encoding halolysin 172P1 from Nab. asiatica (Kamekura et al. 1992) and halolysin R4 from Hfx. Mediterranei (Kamekura et al. 1996) have been identified. Characterization of the biochemical properties in combination with … WebApr 1, 2024 · In this study, a halolysin from the extremely halophilic archaeon Haladaptatus sp. DYF46 (HlyHap) was identified to be a novel type of halolysin without C-terminal … smart building sensors

Biochemical characterization of a low salt-adapted extracellular ...

An extracellular halophilic protease SptA from a halophilic

WebAug 3, 2024 · Halolysin SptA is the major extracellular protease of Natrinema sp. J7-2, and its production is growth-phase dependent. The growth phase-dependent production of halolysin has been described in some haloarchaea such as Ncc. occultus (Studdert et al., 1997), Nab. asiatica (Kamekura and Seno, 1990), and Nab. magadii (Paggi et al., 2010). WebMar 1, 1992 · Halolysin has a long C-terminal extension of approximately 120 amino acids which has not been found in other extracellular subtilisin type serine proteases. The gene, hly, was expressed in another ... smart building show london excelWebYet the diversity of halolysins remains underexplored. In this study, a halolysin from the extremely halophilic archaeon Haladaptatus sp. DYF46 (Hly Hap) was identified to be a … hill the world lyrics

"WebGenerally, HlyA from the extremely halophilic archaeon Hcc. salifodinae is an alkaliphilic and low salt-adapted halolysin with high activity, thus representing an attractive candidate for various industrial uses. " - Halolysin

Halolysin

WebJun 11, 2024 · A gene encoding a halophilic serine proteinase, halolysin R4, from a halophilic archaeon Haloferax mediterranei strain R4 was cloned, its nucleotide sequence determined, and expressed in Haloferax volcanii WFD11. The deduced amino-acid sequence (403 aa in length) showed the highest s:[milarity to halolysin 172P1, produced … WebA novel halolysin without C-terminal extension from an extremely halophilic archaeon Author: Jing Hou, Si-Ya Li, Yang-Jie Zhao, Heng-Lin Cui Source: Applied microbiology and biotechnology 2024 v.106 no.8 pp. 3009-3019 ISSN: 0175-7598 Subject:

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WebJun 9, 2010 · Aims: The alkaliphilic haloarchaeon Natrialba magadii secretes a halolysin-like protease (Nep) that is active and stable in high salt and in organic solvents, which represents a potential resource for biocatalysis in low water activity conditions. In this study, the effect of the growth stage on Nep biosynthesis was examined. Methods and Results: … WebJan 15, 2024 · Sequence (a) and crystal structure (b and c) of a polypeptide chain fragment from the surface layer protein tetrabrachion from Staphylothermus marinus showing the right-handed parallel RHCC tetramer. In (a) the sequence positions are labelled from a to k with the hydrophobic core positions highlighted in red. (b) Axial view from the N-terminus ...

WebHalolysin SptA from haloarchaeon Natrinema sp. J7 consists of a subtilisin-like catalytic domain and a C-terminal extension (CTE) containing two cysteine residues. In this report, we have investigated the function of the CTE using recombinant enzymes expressed in Haloferax volcanii WFD11. Deletion of the CTE greatly reduced but did not abolish ... WebThe gene of a halophilic alkaline serine protease, halolysin, from an unidentified halophilic archaea (archaebacterium) was cloned and its nucleotide sequence was determined. …

WebCiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): The study used various screening techniques, clustering, decision tree and generalized rule induction (association) (GRI) models and molecular phylogenic relationship to search for patterns of halophilicy and to find features contribute to halolysin salt stability. We found that Met … WebJun 14, 2008 · The Hfx. volcanii synthesized protease was active in high salt, high pH and high DMSO. This study provides the first molecular characterization of a halolysin-like protease from alkaliphilic haloarchaea and is the first description of a recombinant system that facilitates high-level secretion of a haloarchaeal protease.

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WebApr 15, 2024 · Except the halolysin from Hbt. halobium, the other characterized halolysins displayed the optimum activities at temperatures higher than 45 °C. The halolysin from Halococcus sp. GUGFAWS-3 exhibited high activities at a very broad temperature range of 40–80 °C. It displayed the optimum temperature of 70 °C, much higher than the optimum ... hill the world testoWebThe gene of a halophilic alkaline serine protease, halolysin, from an unidentified halophilic archaea (archaebacterium) was cloned and its nucleotide sequence was determined. The deduced amino acid sequence showed that halolysin consists of 411 amino acids, with a molecular weight of 41,963. The highest homology was found with thermitase from … smart building research based on bimWebThe alkaliphilic haloarchaeon Natrialba magadii secretes a halolysin‐like protease (Nep) that is active and stable in high salt and in organic solvents, which represents a potential resource for biocatalysis in low water activity conditions. Aims: The alkaliphilic haloarchaeon Natrialba magadii secretes a halolysin‐like protease (Nep) that is active and stable in … smart building services ramsgateWebApr 15, 2024 · Except the halolysin from Hbt. halobium, the other characterized halolysins displayed the optimum activities at temperatures higher than 45 °C. The halolysin from … hill the world michael jackson parolesWebFeb 12, 2024 · Importance: This study shows that halolysin R4 from Haloferax mediterranei provides its host antagonistic and defensive activities against other haloarchaea, which expands our knowledge on the traditional function of haloarchaeal extracellular proteases. Haloarchaeal extracellular serine proteases have been previously discussed as growth … smart building show londonWebFeb 12, 2024 · This study shows that halolysin R4 from Haloferax mediterranei provides its host antagonistic and defensive activities against other haloarchaea, which expands the knowledge of the traditional function of halo archaeal extracellular proteases. This study shows that halolysin R4 from Haloferax mediterranei provides its host antagonistic and … hill therapeuticsWebMay 23, 1996 · @article{Kamekura1996HalolysinRA, title={Halolysin R4, a serine proteinase from the halophilic archaeon Haloferax mediterranei; gene cloning, expression and structural studies.}, author={Masahiro Kamekura and Yukio Seno and Michael Dyall-Smith}, journal={Biochimica et biophysica acta}, year={1996}, volume={1294 2}, pages={ … smart building services michigan