WebThis enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70°C. GoTaq® Hot Start Polymerase also exhibits 5´→3´ exonuclease activity. The enzyme is supplied with a tube of 25mM MgCl 2 to optimize the magnesium concentration in your reactions. GoTaq® Hot Start Polymerase is supplied with 5X ... WebOverview. Generally, a complete polymerase chain reaction (PCR) requires five basic reagents, including DNA template, forward and reverse primers, DNA polymerase, deoxynucleotide triphosphates (dNTPs) and reaction buffer. GenScript provides not only oligo primers synthesis services, but also cost-effective and high-quality Taq DNA …
Easy-Load PCR SuperMix (Green, 2X)(PCR预混液)(D7256-1ml)
WebThis ready-to-use mixture of SYBR Green I, JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer is provided in a 2× concentrate for ease of use. Simply add 25 μL of the 2× mix to DNA template, primers and water. The JumpStart Taq antibody inactivates DNA polymerase at room temperature. WebApr 5, 2024 · Thermostable DNA Taq-polymerase stop assay can detect the preferential position of the G4 –ligand binging within a long PQS-rich genomic DNA fragment. ... Black—10 mM KCl, blue—0.5 µM PDS, red 1 µM—TMPyP4, orange—0.1 µM PhenDC3, green 5 µM BRACO-19. Oligonucleotides for FRET melting assay are shown under the … datasheet windows server 2019
GoTaq® DNA Polymerase Taq Polymerase PCR
WebPremix Ex Taq DNA Polymerase (Perfect Real Time) is a 2X premix specially designed for high-speed, high-sensitivity, real-time PCR (qPCR). It includes a high-fidelity hot-start enzyme and optimized buffer for superior specificity and increased amplification efficiency. WebPolymerase chain reaction, or PCR, is a technique to make many copies of a specific DNA region in vitro (in a test tube rather than an organism). PCR relies on a thermostable DNA polymerase, Taq polymerase, and requires DNA primers designed specifically for the DNA region of interest. In PCR, the reaction is repeatedly cycled through a series ... Web구성 및 보관. • 2 x 1.25 mL Luminaris Color HiGreen High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye; sufficient for 250 x 20 µL reactions. The master mix contains high concentration of ROX passive reference dye for specific real ... bitterfelder recycling