WebDec 12, 2024 · A CRISPR/Cas9-based genome-wide screening was used to identify genes in HUH-7 SR cells that conferred resistance to sorafenib ... (#554), 30 μg psPAX2 (#842) and 25 μg of the GeCKO plasmid library. The plasmids were diluted in a 250 mM CaCl 2 solution. This solution was then mixed (v/v) with 2x HEPES buffer (NaCl 280 mM, KCl 10 … WebThe genome-scale CRISPR knock-out (GeCKO) v2 libraries enable rapid screening for loss-of-function mutations that produce a desired phenotype in human or mouse cells. …
2010003K11Rik CRISPR Knockout and Activation Products (m)
WebPlasmid는 PSCA-특정 20 nt guide RNA sequences으로 구성되였으며 이는 GeCKO (v2) library에서 유래되였습니다. CRISPR 유전자 knockout에 대해 gRNA sequences는 Cas9프로틴을 인도하여 게놈DNA 특정위치에 double strand break (DSB)를 형성합니다. 유전자 녹아웃과 활성화에 타겟 특정 CRISPR ... WebMar 24, 2024 · CRISPR Knock-Out library amplification and lentiviral production Pooled genome-wide CRISPR screen We followed previously published protocols of screens using the GeCKO library, as developed by the ... histone folding dna and cancer
Genome-scale CRISPR-Cas9 knockout screening in human …
WebDec 21, 2024 · Here, we show that our recently-described CRISPRko library (Brunello) is more effective than previously published libraries at distinguishing essential and non-essential genes, providing... WebThis lentiviral CRISPR library targets 3' splice sites with paired guide RNAs. It induces skipping of poison cassette exons and corresponding upstream constitutive exons. ... Mouse GeCKO v2 genome-wide library. Description . Delivered as two half-libraries (A and B) allowing researchers to screen with 3 or 6 gRNAs/gene, respectively. 1000000052 ... WebThe GeCKO v2 libraries consist of over 100,000 unique gRNAs for gene knock-out in either the human or mouse genome. Each species-specific library is delivered as two half … Species - the gRNAs within a given CRISPR library are unique to the … Plasmid lentiGuide-Puro from Dr. Feng Zhang's lab contains the inserts S. … histone extracts