Flp-intm t-rextm 293 细胞系
WebFeb 20, 2024 · 在我们以前的工作中，我们在四环素诱导型CMV启动子（HEK-293 Flp-In T-REx-Thermo）的控制下，建立了表达C末端3xFLAG标记的外来体成 … WebApr 21, 2024 · Flp-In 293 T-REx细胞系是为了快速生成稳定的细胞系而设计的，它可以确保从Flp-In表达载体上获得的蛋白的均匀表达。 这些细胞在转录活跃的基因组位点上包含 …
Flp-intm t-rextm 293 细胞系
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WebFlp-InTM T-RExTM 293 cells stably harboring hFFA2-eYFP at the Flp-InTM T-RExTM locus were induced to express the receptor. The capacity of GLPG0974 to inhibit elevation of [Ca2+] i. produced by EC80 concentrations of C3 or Cmp 1 is shown (B). Membranes from such induced cells were used to assess the ability of GLPG0974 to inhibit the binding of ... WebBioz Stars score, Techniques, Protocol Conditions and more for Flp Intmt Rextm 293 Cells Hek 293t Hek Human Embryonic Kidney 293 Cells, supplied by Thermo Fisher. Data for …
WebFigure 5-9. PMA induces internalisation of hFFA4. Flp-InTM T-RExTM 293 cells able to express FLAG-hFFA4-mVenus were plated down onto poly-D-lysine-coated 30 mm glass coverslips and receptor expression was induced using doxycycline (100 ng/ml). The internalisation of hFFA4 in live cells was then imaged using a Zeiss VivaTome spinning … Web丁香通为您提供FLPINTREX293商品详情介绍：价格：￥1 - 2000，货号：R78007，品牌：invitrogen,GIBCO，产地：美国，详见丁香通FLPINTREX293商品详情页；
WebAbstract. Each subtype of the muscarinic receptor family of G protein-coupled receptors is activated by similar concentrations of the neurotransmitter acetylcholine or closely rel WebFlp-In™ stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows …
WebApr 2, 2024 · 基本信息. 商品名称：Epigenetics in Cancer 作者：编者:JUNJIANG FU//SABER IMANI 定价：198 出版社：科学 ISBN号：9787030543769 其他参考信息 （以实物为准）. 出版时间：2024-01-01 印刷时间：2024-01-01 版次：1 印次：1 开本：16开 包装：平装 页数：364 内容提要
WebThe Flp-InTM T-RExTM 293 cells were generated by cotransfection of the constructed plasmid and pOG44 plasmid to express the target gene. Successfully recombined monoclonal cell lines were screened by hygromycin B resistance. Western blot and indirect immunofluorescence (IFA) were used to examine the expression of the target protein in ... incarnation what is the trinity year 3WebAug 21, 2024 · 最近，有研究通过RMDI技术在Flp-InTM-293细胞中系成功地过表达核孔复合物亚基，以及在Flp-InTM-CHO细胞中表达P2Y12受体。 已有研究构建了具有两个不同重组位点（双重RMCE）的宿主细胞，用于在单个细胞系中过表达多个外源基因。 inclusion\u0027s szWeb293t细胞由293细胞通过基因技术派生出的细胞系，是经过腺病毒e1a基因的转染，能表达sv40大t抗原，含有sv40复制起始点与启动子区。 许多真核表达载体如pcDNA3.1中含有SV40病毒的复制起始位点，可以在表达SV40病毒T抗原的细胞系中复制，从而提高外源基因 … inclusion\u0027s stWebFigure 4-2. TUG-891 but not TUG-1197 displays low potency mediated activation of hFFA1. Flp-InTM T-RExTM 293 cells able to express hFFA1-mVenus-HA were seeded (75,000 cells /well) into poly-D-lysine coated black clear-bottom 96-well plates. Expression of hFFA1 was induced by the antibiotic doxycycline (100 ng/ml) for 18-24 hours. Cells were … inclusion\u0027s swWebC, immunoblots of cell lysates (input) used for FLAG immunoaffinity purification in B. D, MED21 lacking the first 5 amino acids is defective in TNFα-induced gene activation. Firefly luciferase activity was measured in lysates from Flp-InTM T-RExTM-293 cells stably expressing wild type or mutant MED21. incarnation what is the trinityWebSep 29, 2024 · 293FTM细胞是由Flp-InTM T-RExTM 293细胞导入单嗜性（ecotropic）报告质粒和MAPPIT (mammalian protein-protein interaction trap) 报告质粒获得，常用于蛋白互作筛选。 293S 细胞. 293S细胞支持悬浮培养，S为Suspension悬浮的首字母，可以耐受低Ca2⁺培养体系。 incarnation year 3Web技术资料. 规格：. 1ml. Flp-In™系统使您可以稳定整合和表达感兴趣的基因，以提供单拷贝等基因细胞系。. Flp-In™表达会在您选择的哺乳动物细胞系基因组中引入一个Flp重组靶 … inclusion\u0027s sy