Cell-based assay nbd cholesterol
WebEvery one of our CytoSelect™ brand of cell-based assays provides fully quantitative results with no manual cell counting required. We are actively developing new cell-based … WebEnter the email address you signed up with and we'll email you a reset link.
Cell-based assay nbd cholesterol
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WebSep 10, 2024 · NBD cholesterol stock solution was prepared according to a previous method with modification. Caco-2 cells were incubated in Host Based Security System (HBSS) containing NBD cholesterol for 1 h at 37 °C in a humidified atmosphere of 5% CO 2 in air. Cells were washed twice in HBSS to remove any NBD cholesterol that was not … WebObjectives Macrophage cholesterol efflux plays an important role in maintaining cellular lipid homeostasis, and preventing cells from formation of lipid-laden foam cells. Although radioactive [3H]-cholesterol was widely used as a tracer in cholesterol efflux assay, time- and labour-consuming assay procedure and radioactivity disposal procedure may limit …
WebIdentification of ACAT1- and ACAT2-specific inhibitors using a novel, cell-based fluorescence assay: individual ACAT uniqueness J Lipid Res. 2004 Feb;45(2) :378-86. … WebUsing a fluorescent NBD-cholesterol binding assay, we found that all three Aster domains avidly bound sterols (Petrescu et al., 2001. Petrescu A.D. ... After infection, GFP positive …
WebGeneral notes. NBD Cholesterol Staining Dye Kit ab269448 can be used to study the mechanisms and biological factors that regulate cholesterol movement within cells. NBD Cholesterol is a fluorescently labelled cholesterol. This kit uses SomaServe's PolyNaut™ dye-loading to load NBD cholesterol into live cells in cell cultures. WebFirst, cells are labeled with NBD-Cholesterol for 24 h, then equilabrated with serum-free media and testcompounds for 18 h followed by incubation with Apo A1 serving as …
WebNBD cholesterol is an environment-sensitive probe that localizes in the membrane's interior and is useful for investigating lipid transport processes as well lipid …
WebC-12 NBD cholesterol is a fluorescently tagged cholesterol with the hydrophilic NBD fluorophore attached to the hydrophilic end of cholesterol, separated by a 12-carbon spacer. This design allows the cholesterol to properly orient in membrane bilayers while the fluorescent tag is presented outside of the bilayer. ... Cell-Based Assay Buffer ... how to access intel management engineWebThe kit employs NBD Cholesterol, a fluorescently-tagged cholesterol, as a probe for the detection of cholesterol taken up by cultured cells. U-18666A, which increases … how to access instagram without phone numberWebNBD-C6-3-Cholesterol and NBD-C12-3-Cholesterol are fluorescently-tagged cholesterol with the hydrophilic NBD fluorophore attached to the hydrophilic end of cholesterol, separated by a 6- and 12-carbon spacer. This design allows the cholesterol to properly orient in membrane bilayers while the fluorescent tag is presented outside of the bilayer. metal technologies corporateWebSep 30, 2013 · While the US draft guidance urges the use of cell-based assays for determination of NAbs, the European guideline allows the use of a non-cell-based … metal tech newsWebprotocol. Cell-Based Assay U-18666A (Item No. 10009869), a cholesterol transport inhibitor, is included in the kit to be used as a positive control (provided at a … how to access interactive menu gta 5 xbox oneWebAC29 cells (A) and AC29 cells stably transfected with ACAT2 (B) were incubated with 1 g/ml NBD-cholesterol for 120 min. Cells were fixed with formaldehyde as described in Methods and viewed with ... metal technologies new hope mnWebAug 30, 2024 · Dissolve the Cell-Based Assay Buffer Tablet in 100 mL of distilled water. 5.2 Cell-Based Assay NBD Glucose 1. This fluorescently-tagged glucose derivate is supplied as a solution in ethanol at 10 mg/mL (approximately 30 mM). 2. Dilute this fluorescent solution in 1/50-1/100 in the glucose-free metal technologies inc. bloomfield in